Calotropis gigantea latex, antifungal activity, minimum fungicidal concentration, zone of inhibition, disc diffusion method correspondence to author. Phytochemical analysis, antibacterial and antioxidant. Antibacterial activity of crude extract and latex crude was 20. Chromatographic separation of the chloroform extract afforded the known compound. Hepatoprotective activity of calotropis gigantea root bark in experimental 281 hepatoprotective activity of calotropis gigantea root bark experimental liver damage induced by dgalactosamine in rats pradeep deshmukh1, tanaji nandgude1, mahendra singh rathode1, anil midha1, nitin jaiswal2 1department of pharmacology, b r nahata college of pharmacy, mhowneemuch road. Antimicrobial activities evaluation from the extracts of.
This plant is popularly known because it produces large quantity of latex 1. Br asclepiadaceae is a xerophytic, erect shrub, growing widely throughout the tropical and subtropical regions of asia and africa. Extracts on growth and survival dynamics of selected pathogenic microorganisms the growth profiles of c. Antimicrobial potential of calotropis gigantea against a wide range of microorganisms was studied. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of calotropis procera was.
Preliminary phytochemical screening and gc ms issn. This study is collective information concerning the ethnobotany pharmacology, phytochemistry and biological activities of the calotropis gigantea. The medicinal properties of this plant represent it as a valuable source of medicinal compound. The antimicrobial activity of crude aqueous extract of latex of calotropis gigantea apocynaceae was tested against six pathogenic species of bacteria staphylococcus aureus, bacillus cereus. The 11 analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and 12 flower of calotropis procera was carried out in disc method and also determined mic value at 600nm through optical density using spectrophotometer. Study on antibacterial activity of calotropis procera peerj preprints. Calotropis gigantea is an important indian medicinal plant and widely used in ayurveda for management.
A method of preparing biologically active derivatives from calotropis gigantea flowers includes obtaining fresh calotropis gigantea flowers, drying the flesh flowers, soaking the dried flowers in a natural oil, e. The aim of the present study was to screen leaves of. An overview of its phytochemistry and pharmacology shoaib quazi, kumkum mathur, sandeep arora. Calotropis gigantea, family apocynaceae, is a milkweed traditionally used in ayurveda for its antihelminthic, antipyretic, and antimalarial activities. Saini download pdf international journal of pharmaceutical and phytopharmacological research. Calotropis procera belongs to the family aselepiadaceae. Antimicrobial activity of extracts and latex of calotropis. Percentage mortality of mosquito larvae after exposure to various concentrations of nano silver particles. Screening and gcms profiling of ethanolic flower extract of calotropis gigantea linn. In the present study, the antioxidant, antibacterial, antifungal and antitumor potential of its three root extracts viz. Antimicrobial and cytotoxic activity of di2ethylhexyl. Pdf antibacterial activity of aqueous extract of calotropis.
This extract is a naturally available extract which can be used for several indications in dentistry for instance, as an antibacterial root canal obturating material or. Anhydrosophoradiol3acetate, antimicrobial, calotropis gigantea and di2ethylhexyl phthalate the emergence of human pathogenic microorganisms that are resistant to major classes of antibiotics has been increased in recent years, due to the indiscriminate use of antimicrobial drugs karaman et al. The nhexane soluble portion of the chloroform extract showed in vitro antiamoebic activity against the hk9 strain of entamoeba histolytica. T o evaluate possible anxiogenic activity, sedative property and anxiolytic potential of crude ethanolic extract of calotropis gigantea leaves. Calotropis gigantea linn and calotropis procera ait 5.
Antimicrobial activity of extracts and latex of calotropis procera ait. Pdf antimicrobial activity of akanda calotropis gigantea. Antibacterial activity of aqueous extract of calotropis. Studies on endophytic fungi of calotropis procera l. Antiasthmatic activity calotropis gigantea showed anti asthmatic activity in ova albumin ova induced asthma. In ancient ayurvedic medicine the plant calotropis gigantea is known as sweta arka. Calotropis procera extracts against seedborne fungi agrees with the work of abdulmoniem et al. Evaluation of antibacterial activity of calotropis. Sherman, microbiology a laboratory manual, benjamin. Antimicrobial activity and phytochemical analysis of. Aqueous leaves extract of calotropis gigantea showed antidengue activities in human whole blood samples. Out of all the plant extracts, calotropis gigantea leaf extract was showing promising qsi activity against pathogenic bacteria pseudomonas. A downloadable pdf document of a book in prepublication awaiting illustration.
Medicinal plants are important source of potentially useful structures for the development of novel chemotherapeutic agents. Both fresh as well as aqueous extracts of dried latex exhibited a dosedependent inhibition of spontaneous motility paralysis and evoked responses to pinprick. The zones of inhibition were measured and recorded. Received application of latex of calotropis gigantea 200 mgkgday wound healing activity excision and incision wound models were used to evaluate the woundhealing activity of latex of calotropis gigantea. Pdf antibacterial activity of aqueous extract of calotropis gigantea. Hepatoprotective activity of calotropis gigantea root bark. Exclusive spectroscopic and microscopic studies were assisted for the physiochemical characterization of agnps. Antimicrobial activity of flower extracts of calotropis gigantea. Antimicrobial activity of methanolic plant extracts against test bacteria. Antimicrobial activity of latex silver nanoparticles using. Pdf antimicrobial activity of leaves and latex extracts of.
Green synthesis of silver nanoparticles using calotropis. For the fungi, the test was carried out on sda plates and incubated at 30oc for 72 hours. Antimicrobial activities of calotropis procera 107 open wells were filled with 0. Antifertility activity the effect of an ethanolic extract of the roots of calotropis procera has been studied in albino rats to explore its antifertility and hormonal activities. Calotropis gigantea is a common wasteland weed and known for various medicinal properties. Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera. This extract is a naturally available extract which can be used for several indications in dentistry for instance, as an antibacterial root canal obturating material or an antiseptic mouthwash. Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i. Sedative and anxiolytic effects of ethanolic extract of. Result of the antimicrobial activity of root extracts of calotropis gigantea. Abstractcalotropis procera and calotropis gigantea are medicinal plant having therapeutic value. Extracts from the root bark of calotropis gigantea were subjected to bioactivityguided fractionation using growth inhibitory effects against entamoeba histolytica. The study was approved by the institutional animal ethical committee of b.
The larvicidal and antimicrobial activity against gram positive and gram negative bacteria were analysed. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity. The crude latex of calotropis procera was evaluated for anthelmintic activity using adult earthworms. Anthelmintic activity of latex of calotropis procera. Different parts of the plant have immense potential to. Antimicrobial activity of green silver nanoparticles from.
Flow cytometric evaluation of calotropis gigantea for. The fresh dried leaves of the plant calotropis gigantea were successively extracted. Activity of extracts and procesterol from calotropis. Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology, university of science and technology bannu, khyber pakhtunkhwa pakistan key words. Quorum sensing inhibitory activity of calotropis gigantea. On the other hand, the extracts obtained calotropis procera seeds tested have been evaluated for their possible in vitro. Antimicrobial activity of solvent extracts and flavonoids of calotropis procera growing wild in saudi arabia was evaluated using the agar welldiffusion method. Steroids and cardenolides exhibited maximum inhibitory activity against f. Invitro antimicrobial activities and phytochemical screening. Antibacterial activity of sample were tested by using 30. The anthelmintic activity of calotropis procera linn. Antimicrobial activities of different parts of calotropis. Calotropis acts as a purgative, anthelmintic, anticoagulant, palliative in problems with respiration, blood pressure, antipyretic, and analgesic, and induces neuromuscular blocking activity.
Antimicrobial activity of hexane extracts of plants against test bacteria. Study on antibacterial activity of calotropis procera. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria. Antimicrobial activity of latex of calotropis gigantea against pathogenic. Pdf calotropis gigantea is a common wasteland weed and known for various medicinal properties. Antimicrobial and cytotoxic activity of di 2ethyl hexyl phthalate and anhydrosophoradiol 3acetate isolated from calotropis gigantea linn. Evalution of anti microbial activity and phytochemicals 51 materials and methods collection of plant material. Antimicrobial activities of calotropis procera on selected. Introduction the potential of higher plants as source for new drugs is still largely unexplored. Conclusion plant mediated green synthesis of silver nanoparticles using calotropis gigantea leaf extract was done. Phytochemical and pharmacological studies of the whole plant. It has also been studied widely for its anticancer potential. Flow cytometric evaluation of calotropis gigantea for determining its antimicrobial activities in infected human whole blood samples. The present study is related to evaluate antimicrobial activities of chloroform and methanol extracts of seeds of calotropis procera located in the forest area of ghaziabad, india.
Antifungal activity of leaf and latex extracts of calotropis. Extraction and phytochemical investigation of calotropis. To test of antimicrobial activity, disc diffusion method has been followed. Cnsdepressant, anxiolytic, calotropis gigantea, locomotor activity objective. The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of calotropis procera and calotropis gigantea and characterization of their bioactive metabolites by fourier transform infrared ftir spectroscopy and gas chromatographymass spectroscopy gcms. Antibacterial and cytotoxic potential of silver nanoparticles. But a few of isolated compounds from calotropis gigantea and amoora rohituka have potent antineoplastic, antimicrobial and insecticidal activities.
Preliminary phytochemical screening of calotropis gigantea. The aim of this investigation was to carry out the analysis of antibacterial and antifungal activities of different parts of calotropis gigantea. Phytochemical and antimicrobial evaluation of aqueous and. Antimicrobial activity of ethanol, methanol and chloroform extracts from flower, leaves and roots of c. Antifungal, antioxidant and antibacterial activities of calotropis procera mushtaq ahmed, rehmat ali khan, s. Pdf antimicrobial activity of latex of calotropis gigantea against. Little research has been done to study the electrophysiological effects of this plants extracts on cardiac, smooth, and skeletal muscle activities. Evaluation of antibacterial activity of calotropis gigentica. Bacillus subtilis mtcc 121, staphylococcus aureus mtcc 96, pseudomonas aeruginosa mtcc 429, escherichia coli. The ethyl acetate extract of this plant showed significant showed significant antibacterial and antifungal effect against most of the pathogenic organic organisms. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli. Antibacterial and phytochemical analysis of stem and root.
A phytoehemieal study on the flowe r of calotropis gigantea linn. These plant products can be derived from barks, leaves, flowers, roots, fruits, and seeds. Antimicrobial activity of green silver nanoparticles from endophytic fungi isolated from calotropis procera ait latex nadia hussein mohamed 1,2, mady ahmed ismail 1, wael mostafa abdelmageed 3,4, ahmed abdelfattah mohamed shoreit 1. Apocynaceae species often contain a mixture of cardiac glycosides, including calactin, uscharin, calotoxin, and calotropin. Calotropis gigantea crude extract, phytochemicals, antimicrobial activity. Pdf antimicrobial activity and phytochemical analysis of.
Alamer3 and abdul aziz bahobail3 1molecular biotechnology research unit, department of biotechnology, faculty of. Calotropis gigantea, well plate method, antibacterial activity. Antimicrobial activity of flower extracts of calotropis. Synthesized agnps were evaluated for its antimicrobial and cytotoxicity potential under invitro conditions. The leaves are broadly elliptical to oblongobovate in shape, with the size of 9. Antimicrobial activity of calotropis procera seeds. The structures of these compounds were confirmed by spectroscopic analyses ir, hrtofms and nmr. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study gaurav kumar, loganathan karthik and kokati venkat bhaskara rao molecular and microbiology research lab, environmental biotechnology division, school of bio science and technology, vit university, vellore632 014, tamil nadu, india. Collection of plant material the leaves and fruits of calotropis gigantea also known as crown flower. Antifungal, antioxidant and antibacterial activities of. The anxiogenic activity of crude ethanolic extract of calotropis gigantea leaves was. This study attempts to determine the phytochemical analysis and antimicrobial effect of c.
An recent approach on calotropis gigantea and calotropis procera. Chloroforms extract of calotropis procera seeds exhibited better antimicrobial activity. College, patna, magadh university, bihar, india corresponding author a b s t r a c t keywords calotropis e pharmacology, m enumerates the morphology and ethno. The plant is erect, tall, large, much branched and perennial with milky latex throughout. Calotropis gigantea linn apocynaceae is a glabrous or hoary, laticiferous shrubs or small trees, about 34 m tall commonly known as the swallowwort or milkweed. The study emphasizes a novel route to synthesis agnps using latex aqueous extract of calotropis gigantea l. Activity of calotropis gigantea madhurima dutta, sarmistha rej. The effect of calotropis gigantea at 100, 200, 400 mgkg, on different body cells, enzymes and histopathological changes were observed.
Antioxidant, antimicrobial activities and gcms analysis of calotropis gigantea white flowers shopna rajamohan, prabakaran kalaivanan, ilayaraja sivagnanam, manivannan rajamanickam abstract calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed. Calotropis procera, latex, bioactive, antimicrobial activity, minimum inhibitory concentration. Pdf antimicrobial activity of leaves and latex extracts. Methanolic root extract of calotropis g igantea induces. Evaluation of anti microbial activity and phytochemical. Many recent reports were published on biosynthesis of agnps using plant latex11, 12, natural rubber latex14, 15, plant extract or by the whole plant showing promising biological activities 6, 16, such as cytotoxic and antimicrobial activities. Efficacy of antibacterial activity of calotropis gigantea. Calotropis gigentica has shown antimicrobial activity against s. Calotropis procera, latex, antimicrobial, antioxidant, antifungal. Maximum inhibitory activity was observed in ethanolic extracts of root bark against enterobacter cloacae and of the stem against f. Evalution of anti microbial activity and phytochemical. The present study was aimed to investigate the phytochemical screening and antimicrobial activities of.
The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. The latex of these plants has been used in africa as an arrow poison. Antimicrobial activity of akanda calotropis gigantea l. Antioxidant, antimicrobial activities and gcms analysis of. Calotropis procera possesses antinociceptive activity, which is independent of the opioid system s aber et al. Antimicrobial activities evaluation from the extracts of leaves, flowers, fruits and latex of calotropis procera from taif abdel aziem farouk1,2, n. It is a wellknown herbal drug used to treat diversified physiological conditions. Medicinal plants are considered to be safer, nontoxic and less harmful as compared to synthetic based drugs that are available. It has been found that the light radiation affects the antimicrobial activity of herbal products. Pdf antimicrobial activity of latex of calotropis gigantea. An excellent, if rather terse, guide to the traditional medicinal uses of the plants of the region in nepal, the latex is applied to sprains, body pains, boils and pimples. Antimicrobial and phytochemical activity, calotropis gigantea,s.
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